Genetic loci and candidate genes with pleiotropic effects for tofu and soymilk weights across multiple environments in soybean (Glycine max) |
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Authors: | Meihong Huang Shiliang Chen Wenlong Li Rui Tian Hua Zhang Zhenqi Shao Xihuan Li Caiying Zhang |
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Institution: | 1. State Key Laboratory of North China Crop Improvement and Regulation, Baoding, Hebei, China
North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Baoding, Hebei, China
Key Laboratory for Crop Germplasm Resources of Hebei, Baoding, Hebei, China
Hebei Agricultural University, Baoding, Hebei, China;2. State Key Laboratory of North China Crop Improvement and Regulation, Baoding, Hebei, China |
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Abstract: | Soybean provides large amounts of protein for human, and the most popular consumption pattern is tofu and soymilk. Recently, the demand of tofu and soymilk is increased, and the breeding of special variety becomes very urgent. To illustrate the genetic basis of these traits, 269 accessions were evaluated under six environments, and 32 associated SNPs were identified on nine chromosomes across multiple environments or conferring diverse traits. Of these SNPs, 16 were demonstrated by BLUP values. Seven adjacent-SNPs were first identified to associate with tofu and soymilk weights in an 1.02-Mb region on chromosome 8, which were verified by BLUP-values. Meanwhile, four neighbouring SNPs in a 0.32-Mb region on chromosome 12 were first identified to associate with these traits across multiple environments, of which three were detected in BLUP values. Furthermore, some candidate genes, that is, α-glucan water dikinase Glyma.08G283700 and E3 SUMO-protein ligase Glyma.12G071300, were screened out with differential expressions between the high- and low-tofu/soymilk-output accessions. Thus, these associated SNPs and candidate genes provide new insight into the genetic basis of tofu and soymilk processing quality in soybean. |
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Keywords: | candidate genes genetic loci GWAS soybean tofu and soymilk |
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