A new approach to Fourier transform Raman: Identification of haploids in maize (Zea mays) |
| |
| Authors: | Jocimar Costa Rosa Renan Santos Uhdre Marcos Ventura Faria Ronald José Barth Pinto Robson Marcelo Rossi Antonio Medina Neto Carlos Alberto Scapim |
| |
| Institution: | 1. Departamento de Genética e Melhoramento, Universidade Estadual de Maringá, Maringá, Paraná, Brazil;2. Departamento de Agronomia, Universidade Estadual do Centro-Oeste, Guarapuava, Paraná, Brazil;3. Departamento de Estatística, Universidade Estadual de Maringá, Maringá, Paraná, Brazil;4. Departamento de Física, Universidade Estadual de Maringá, Maringá, Paraná, Brazil |
| |
| Abstract: | The objective of this work was to adapt the FT Raman spectroscopy analysis in the differentiation of haploid and diploid kernels in maize, developing a new efficient, agile, precise, and nondestructive methodology. The main difference observed in FT Raman readings was a peak in the region between 1600 and 1700 cm?1 in possibly haploid kernels. It was possible to correlate the characteristics of the kernels with the presence of the R1-nj gene and the readings obtained in the Raman spectrometry technique. Most of the kernels previously classified as haploid showed positive values for principal component analysis (PCAs), indicating a correlation in the identification of haploids by the techniques adopted. The identification of haploids by R-Navajo was superior to FT Raman. However, FT Raman spectroscopy is an agile analysis technique that enables the development of non-invasive and non-destructive analytical methods in maize kernels, in addition to providing relevant information about the chemical structures present in the composition of the samples. |
| |
| Keywords: | double-haploid lines flow cytometry Zea mays L |
|
|
