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| 新城疫病毒xx08毒株血凝素-神经氨酸酶基因主要抗原区原核表达及鉴定 | |
| 引用本文： | 朱艳平,田献礼,李鹏,岳锋,贾文科,张艳芳,孙国鹏,郭东光,刘卫,王选年. 新城疫病毒xx08毒株血凝素-神经氨酸酶基因主要抗原区原核表达及鉴定[J]. 河南农业科学, 2012, 41(7): 134-137,154 |
| 作者姓名： | 朱艳平 田献礼 李鹏 岳锋 贾文科 张艳芳 孙国鹏 郭东光 刘卫 王选年 |
| 作者单位： | 1. 新乡学院生命科学与技术系生物技术研究中心,河南新乡,453003 2. 河南科技学院动物科学学院,河南新乡,453003 3. 新乡学院生命科学与技术系生物技术研究中心,河南新乡453003;河南科技学院动物科学学院,河南新乡453003 |
| 基金项目： | 教育部科学技术研究重点项目(207065);河南省高校科技创新团队支持计划项目(2008IRTSTHN011);河南省基础与前沿技术研究项目(330002) |
| 摘 要： | 利用基因工程技术构建新城疫病毒HN基因抗原表位集中区HNI175-K367基因片段(523-1101位)的重组表达质粒pET32-HNI175-K367。将该质粒转化大肠杆菌BL21(ED3),经IPTG诱导,SDS-PAGE鉴定表明,HNI175-K367蛋白得到高效表达,其分子量约为38kD。Western-blot分析证实,HNI175-K367蛋白与鸡新城疫病毒高免血清发生特异性反应,表明利用原核表达系统获得的重组蛋白具有良好的反应原性。 |
| 关 键 词： | 新城疫病毒 HN蛋白抗原表位集中区 原核表达 多克隆抗体 鉴定 |
Prokaryotic Expression of the Main Antigen Region in HN Gene of Newcastle Disease Virus xx08 Strain |
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| ZHU Yan-ping , TIAN Xian-li , LI Peng , YUE Feng , JIA Wen-ke , ZHANG Yan-fang , SUN Guo-peng , GUO Dong-guang , LIU Wei , WANG Xuan-nian. Prokaryotic Expression of the Main Antigen Region in HN Gene of Newcastle Disease Virus xx08 Strain[J]. Journal of Henan Agricultural Sciences, 2012, 41(7): 134-137,154 | |
| Authors: | ZHU Yan-ping TIAN Xian-li LI Peng YUE Feng JIA Wen-ke ZHANG Yan-fang SUN Guo-peng GUO Dong-guang LIU Wei WANG Xuan-nian |
| Affiliation: | 1,2*(1.Biotechnology Research Center,Life Science and Technology Department,Xinxiang University,Xinxiang 453003,China; 2.College of Animal Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China) |
| Abstract: | In this study,the HNI175-K367 gene fragment of HN gene epitope concentration region of Newcastle disease virus(NDV) was cloned into pET-32a(+) vector to construct the expression plasmid pET32-HNI175-K367.The plasmid was transformed into E.coli BL21(ED3) and induced by IPTG for HNI175-K367 protein expression.Then,the expressed protein was separated by SDS-PAGE and identified by western blot assay.The results showed that the HNI175-K367 protein was overly expressed and the molecular weight was about 38 kD.The expressed protein specifically reacted with anti-NDV antiserum,indicating that the prokaryotically expressed target protein had good antigencity. |
| Keywords: | NDV HN protein epitope concentration region prokaryotic expression polyclonal antibody identification |
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