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| 用AFLP技术分离辣椒mtDNA中与雄性不育相关的基因片段 | |
| 引用本文： | 吴智明,胡开林,陈晓莹,乔爱民.用AFLP技术分离辣椒mtDNA中与雄性不育相关的基因片段[J].核农学报,2010,24(1):20-24. |
| 作者姓名： | 吴智明 胡开林 陈晓莹 乔爱民 |
| 作者单位： | 1. 仲恺农业工程学院农业与园林学院,广东,广州510225;华南农业大学园艺学院,广东,广州,510642 2. 华南农业大学园艺学院,广东,广州,510642 3. 仲恺农业工程学院农业与园林学院,广东,广州510225 |
| 摘 要： | 采用AFLP技术对辣椒胞质雄性不育系‘北A’、相应保持系‘北B’及其杂种F1的m tDNA进行了比较分析,获得了9条差异谱带,对全部差异谱带进行了克隆测序和同源性比对分析。结果表明,部分序列同烟草NADH脱氢酶亚基1和核糖体蛋白L2基因、矮牵牛线粒体嵌合基因nad4L-orf25等核苷酸序列存在90%以上的相似性。推断这些差异片段可能与辣椒细胞质雄性不育相关。 |
| 关 键 词： | 辣椒 细胞质雄性不育 线粒体DNA AFLP |
| 收稿时间： | 2009-06-03 |
ISOLATION OF mtDNA FRAGMENTS ASSOCIATED TO CMS IN HOT PEPPER BY AFLP TECHNIQUE |
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| WU Zhi-ming,HU Kai-lin,Chen Xiao-ying,QIAO Ai-min.ISOLATION OF mtDNA FRAGMENTS ASSOCIATED TO CMS IN HOT PEPPER BY AFLP TECHNIQUE[J].Acta Agriculturae Nucleatae Sinica,2010,24(1):20-24. | |
| Authors: | WU Zhi-ming HU Kai-lin Chen Xiao-ying QIAO Ai-min |
| Abstract: | Mitochondrial DNA (mtDNA) from pepper CMS line ‘NorthA’, maintainer line ‘NorthB’ and their hybrid were analyzed and compared by AFLP technique. 9 fragments were obtained. All of the 9 specific fragments were cloned and sequenced. BLAST analysis in GenBank revealed that partial sequences shared above 90% identity with NADH dehydrogenase subunit 1, ribosomal protein L2 Nicotiana tabacum: nad4L-orf25 intergenic sequence of Petunia × hybrida mitochondrial DNA at nucleotide level. It was speculated that the specific fragments might be related to CMS in pepper. |
| Keywords: | AFLP pepper (Capsicum annuum L ) cytoplasmic male sterility mtDNA AFLP |
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