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Detection of Mycobacterium tuberculosis complex antibodies in free-ranged wild boar and wild macaques in selected districts in Selangor and reevaluation of tuberculosis serodetection in captive Asian elephants in Pahang,Peninsular Malaysia
Authors:Yusuf Madaki LEKKO  Azlan CHE-AMAT  Peck Toung OOI  Sharina OMAR  Dhabitah Tatiyana MOHD-HAMDAN  Liya Syahila LINAZAH  Zakirawaranis ZAKARIA  Siti Zubaidah RAMANOON  Mazlina MAZLAN  Faez Firdaus Abdullah JESSE  Mohd Firdaus Ariff ABDUL-RAZAK  Sabri JASNI  Norhamizah ABDUL-HAMID
Abstract:Tuberculosis (TB) is a chronic inflammatory and zoonotic disease caused by Mycobacterium tuberculosis complex (MTBC) members, affecting several domestic animals, wildlife species and humans. The preliminary investigation was aimed to detect antibody against MTBC among indigenous wildlife which are free-ranged wild boar, free-ranged wild macaques and captive Asian elephants in selected areas of Selangor and elephant conservation centre in Pahang, respectively. The results indicate that MTBC serodetection rate in wild boar was 16.7% (7.3–33.5 at 95% confidence interval (CI)) using an in-house ELISA bPPD IgG and 10% (3.5–25.6 at 95% CI) by DPP®VetTB assay, while the wild macaques and Asian elephant were seronegative. The univariate analysis indicates no statistically significant difference in risk factors for sex and age of wild boar but there was a significant positive correlation (P<0.05) between bovine TB in dairy cattle and wild boar seropositivity in the Sepang district.
Keywords:antibody  ELISA  Mycobacterium tuberculosis complex  wild boar  wildlife
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