| Abstract: | Monoclonal antibodies (mAb) against the main photo-addition products of chlorothalonil with olefinic compounds of plant cuticles were produced. An indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of free and bound chlorothalonil and its derivatives. For the characterization of the binding properties of the mAb, derivatives of chlorothalonil (simulating structures of cuticle bound residues) were synthesized. The cross-reactivities of these products were determined by ELISA. The test system was employed to detect bound residues of chlorothalonil in enzymatically isolated tomato cuticles, which had been spiked with methanolic solutions of the compound, irradiated by simulated sunlight and extracted. The use of isolated cuticles allows work to be carried out with authentic material without disturbance by metabolic processes. © 1999 Society of Chemical Industry |
