| Abstract: | Hairy root cultures have been derived from neem (Azadirachta indica A Juss, Family Meliacceae) using Agrobacterium rhizogenes and have been studied for the production of compounds with antifeedant effects on insects. Six-week-old hairy root cultures were extracted, and HPLC yielded fractions ranging from polar to non-polar compounds. High antifeedancy levels against the desert locust were observed in fractions(F) 2, 3 and 4 whilst F1 and F5 were not significantly antifeedant. Interestingly F3 did not contain any of the well-known neem chemicals while F2 contained azadirachtin and 3-tigloylazadirachtol and F4 nimbin and salannin. © 1999 Society of Chemical Industry |
