Shiga toxin 2eB-transgenic lettuce vaccine: N-glycosylation is important for protecting against porcine edema disease |
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Authors: | Takashi HAMABATA Toshio SATO Eiji TAKITA Takeshi MATSUI Takahiro KAWABATA Taishi IMAOKA Nobuo NAKANISHI Takamitsu TSUKAHARA Kazutoshi SAWADA |
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Affiliation: | 1)Research Institute, National Center for Global Health and Medicine, 1-21-1 Shinjuku, Tokyo 162-8655, Japan;2)Advanced Technology Research Laboratories, Idemitsu Kosan Co., Ltd., 1280 Kamiizumi, Sodegaura, Chiba 299-0293, Japan;3)KYODOKEN Institute, 585 Shimoitabashi, Kyoto 612-8073, Japan;4)Kyoto Institute of Nutrition & Pathology, 7-2 Furuikedani, Tachikawa, Ujitawara, Kyoto 610-0231, Japan |
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Abstract: | Porcine edema disease (ED) is a life-threatening toxemia caused by enteric infection with Shiga toxin 2e (Stx2e)-producing Escherichia coli (STEC) in weaned piglets. We previously reported that the stx2eB-transgenic lettuce 2BH strain shows potential for use as an oral vaccine candidate against ED. However, the 2BH strain expressed a hemagglutinin (HA)-tag together with Stx2eB and contained non-canonical N-glycosylation. Therefore, we developed two Stx2eB-lettuce strains, the 3 (G+) strain in which the HA-tag was removed from 2BH, and the 3 (G-) lettuce strain, in which the 73rd Asn was replaced with Ser to prevent non-canonical N-glycosylation of Stx2eB from the 3 (G+) strain. We examined the protective effect of these newly developed two strains compared with the previous 2BH strain against ED using a colostrum-deprived piglet STEC infection model. We found that the N-glycosylated 2BH and 3 (G+) strains relieved the pathogenic symptoms of ED in STEC-challenged piglets, whereas the non-glycosylated 3 (G-) strain did not. N-Glycosylation of the Stx2eB product in lettuce may be involved in the immune response in piglets. |
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Keywords: | colostrum-deprived piglet infection model glycosylation porcine edema disease Shiga toxin 2e-producing Escherichia coli stx2eB-transgenic lettuce vaccine |
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