Abstract: | Soils were collected from a potato-growing area in Serres, Northern Greece, where the nematicide ethoprophos was reported to have lost its effectiveness against cyst nematodes following 30 years of regular use. Incubation studies with ethoprophos and two bioassays using root-knot nematodes demonstrated that, in this heavily treated soil, the nematicide was degraded rapidly and nematicidal activity persisted only up to 14 days. In soil from an adjacent field with no known history of nematicide use during the preceding 14 years, ethoprophos was degraded more slowly and retained its nematicidal activity for more than 35 days. Ethoprophos efficacy was extended when the soil that had been treated in the field was autoclaved, although the effect was only transitory. The addition of ‘pre-conditioned’ soil from the previously treated field to samples of soil from the previously untreated field resulted in a significant acceleration of ethoprophos degradation compared with that observed in unamended soil from the previously untreated field. © 1999 Society of Chemical Industry |