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Enhanced overall resistance to Fusarium seedling blight and Fusarium head blight in transgenic wheat by co-expression of anti-fungal peptides |
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| Authors: | Zheng-Wei Liu He-Ping Li Wei Cheng Peng Yang Jing-Bo Zhang An-Dong Gong Yan-Ni Feng W. G. Dilantha Fernando Yu-Cai Liao |
| Affiliation: | 1. Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China 2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China 3. College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China 4. Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada 5. National Center of Plant Gene Research (Wuhan), Wuhan, 430070, People’s Republic of China |
| Abstract: | Introduction of alien genes into wheat has been proposed as a strategy to breed cultivars with improved resistance to Fusarium seedling blight (FSB) and Fusarium head blight (FHB). In this study, we co-transformed different anti-fungal peptides (AFPs) into an elite wheat cultivar Yangmai11. We identified the genetically stable transgenic wheat lines carrying single or multiple genes by PCR, qRT-PCR and Southern blot analyses. Transgenic wheat lines 451 and 513 expressing two AFPs displayed a consistent, significantly improved overall resistance to FSB and FHB, whereas only FHB resistance was observed from other lines. Furthermore, crude proteins extracted from the lines 451 and 513 showed a clear inhibitory activity against F. graminearum in vitro. Taken together, it was essential to properly combine and express AFPs in transgenic wheat in order to obtain an improved overall resistance to Fusarium pathogens. |
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