First report on molecular surveillance based on duplex detection of Anaplasma marginale and Theileria annulata in dairy cattle from Punjab,Pakistan

The study aimed to investigate if vegetable-based high-energy mash diets supplemented with NaHCO₃, l-arginine?+?vitamin C, and vegetable oils were effective against tachycardia and polycythemia in the broiler chicken. A total of 256 Ross-308 day-old male broiler chicks were randomly distributed into eight dietary treatment groups in a three-way ANOVA with 2?×?2?×?2 factorial arrangement (three factors, i.e., NaHCO₃, l-arginine?+?vitamin C, and vegetable oil each with two levels, e.g., 0 and 0.1% of NaHCO₃ and l-arginine?+?vitamin C; 3 and 4% of vegetable oil supplemented with basal diet) for a period of 35 days. Iso-caloric and iso-nitrogenous diets were formulated and supplied ad libitum. The final live weight (FLW), average daily feed intake (ADFI), average daily gain (ADG), feed efficiency (FE), carcass traits, cardio-pulmonary morphometry, total protein (TP), hemoglobin (Hb), triiodothyronine (T₃), incidence of tachycardia, and polycythemia were examined. Supplementation of NaHCO₃ increased 2.2% ADFI, 5.5% FE, and 23.2% TP. The l-arginine?+?vitamin C increased 2.4% FLW and decreased 1.9% heart rate. Vegetable oil increased 1.3% ADFI, 4.2% ADG, 8.6% FE, 23.1% Hb, and 15.5% PCV. The NaHCO₃, l-arginine?+?vitamin C, and vegetable oil additively interacted to increase 31.5% T₃ at the expense of 21.1% of the weight of the right ventricle (RV). The RV:TV, carcass traits, and hemato-biochemical indices remained within normal range irrespective of the levels of the supplementations of the test ingredients. It was concluded that vegetable-based high-energy mash diets were not susceptible to tachycardia and polycythemia. The addition of NaHCO₃ and l-arginine?+?vitamin C ameliorated the propensity of tachycardia and polycythemia without deteriorating performance, carcass traits, and hemato-biochemical indices of the broiler chicken in a dose-dependent manner.