| Cre／loxp位点特异性重组系统在转基因植物中的应用 |
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| 引用本文： | 邱淑萍,陈在杰,王锋.Cre／loxp位点特异性重组系统在转基因植物中的应用[J].福建农业学报,2008,23(2). |
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| 作者姓名： | 邱淑萍 陈在杰 王锋 |
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| 作者单位： | 1. 福建省农业科学院福建农业遗传工程重点实验室,福建福州,350003;福建师范大学生命科学学院,福建福州,350007
2. 福建省农业科学院福建农业遗传工程重点实验室,福建福州,350003 |
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| 摘 要： | 位点特异性重组系统能够在植物遗传转化中更准确、可靠的操纵外源DNA的引入或删除,已成为植物遗传操作中的重要工具。本文简要介绍目前应用最为广泛的Cre/loxp位点特异性重组系统的重组机制,并着重阐述Cre/loxp系统在删除转基因植物中标记基因及定点整合等方面的应用。
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| 关 键 词： | 位点特异性重组 Cre/loxp 转基因植物 基因删除 定点整合 |
Application of Cre/loxp site-specific recombination technology on transgenie plants |
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| QIU Shu-ping,CHEN Zai-jie,WANG Feng.Application of Cre/loxp site-specific recombination technology on transgenie plants[J].Fujian Journal of Agricultural Sciences,2008,23(2). |
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| Authors: | QIU Shu-ping CHEN Zai-jie WANG Feng |
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| Abstract: | Site-specific recombination technology has been developed and widely applied for plant genetic manipulations.It can successfully delete marker genes from a transgenic plant or site-specifically integrate a gene to a pre-determined genomic location in a precise,single-copy pattern.This article reviews the Cre/loxp site-specific recombination technique and its applications for marker gene deletion and precise gene integration. |
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| Keywords: | site-specific recombination Cre/loxp transgenic plant gene deletion site-specific integration |
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