温室黄瓜产量相关农艺性状QTLs的定位

【目的】秋冬茬和冬春茬是目前中国日光温室黄瓜栽培的两种重要茬口,对两茬黄瓜产量相关性状的QTLs进行定位,为温室黄瓜产量分子标记辅助选择的研究提供理论依据。【方法】选用欧洲8号×秋棚自交系的113份黄瓜重组自交系(RILs)群体作为试验材料,并利用该群体已经构建的包含182个标记的分子连锁图谱对与产量相关的9个性状进行QTL分析。【结果】共检测到58个QTLs,其中与单株平均产量相关的QTL1个,定位于LG4连锁群上;控制黄瓜日增重量的QTL位点6个,分别位于LG2、LG3、LG6连锁群上;控制平均单瓜重的QTL位点5个,分别位于LG1和LG5连锁群上;控制坐瓜数的QTLs2个,位于LG2和LG4连锁群上;控制化瓜率的QTL1个,位于LG7连锁群上;控制第一雌花节位的QTLs28个,在1-8个连锁群上都有分布;控制总叶片数的QTLs8个,分别位于LG2、LG7和LG4连锁群上;控制叶面积的QTLs2个,分别位于LG1和LG3连锁群上。以上产量相关性状的QTLs仅在一个茬口中被检测到。控制雌花总数的QTLs5个,全部位于LG2连锁群上,其中ffa2a、ffa2b是两个茬口共有的QTLs,并且其遗传效应方向一致。研究还发现若干QTL富集区域和成束分布的QTLs。【结论】本项研究共检测到温室黄瓜与产量相关的9个性状的58个QTLs,其中ffa2a、ffa2b在两个栽培环境中表达稳定。

Mapping and Analyzing QTLs of Yield-Associated Agronomic Traits of Greenhouse Cucumbers

Objective]Autumn-winter and winter-spring are two important cropping seasons for cultivating cucumber (Cucumis sativus L.) in solar greenhouses in China.This study was made to map and analyze some candidate QTLs of yield-assoeiated agronomic traits in cucumber,so as to provide information for molecular marker assisted selection of yield of greenhouse cucumber.Method]A population of 113 cucumber recombinant inbred lines(RILs)derivcd from a cross between Europe 8 and Qiupeng was used as materials in this study.Based on the RIL molecular linkage map consisting of 182 markers constructed previously,candidate QTLs conferring 9 yield-associated traits were analyzed.Result]A total of 58 QTLs were detected in this study.Among them.1 QTL conferring single plant yield(SPY) was located on the linkage group LG4;6 QTLs conferring daily gain weight(DAW)were located on LG2,LG3 and LG6;5 QTLs conferring single fruit weight(SFW)were located on LG1 and LG5;2 QTLs conferring the number of fruit setting (NFS) were located on LG2 and LG4;1 QTL conferring fruit abortion rate(FAR)was located on LG7;28 QTLs conferring first female flower node(FFN) were located on LG1 through to LG8;8 QTLs conferring total humber of leaves(TNL) were located on LG2,LG7,and LG4;2 QTLs conferring leaf areas(LA) were located on LG1 and LG3.The above-mentioned QTLs were detected in only one cropping season.There were 5 QTLs conferring female flower number (FFN)on LG2,of which,ffn2a and ffn2b,were detected in both cropping seasons,and with the same positive genetic effects.Some concentrated regions and cluster distribution of QTLs were also found in this study.Conclusion]A total of 58 candidate QTLs in a greenhouse-cultivated cucumber populmion were detected in the study.The two QTLs,ffn2a and ffn2b,were found to be expressed consistently under both cropping seasons of the greenhouse cultivation envkonment.