海南香蕉褐缘灰斑病原菌的核酸分析鉴定

采用种的特异性引物,通过聚合酶链式反应(PCR)方法,对11个海南香蕉褐缘灰斑病菌菌株进行了鉴定。结果表明,11个菌株中,来自海南儋州、乐东、文昌、东方、澄迈、临高、琼海、昌江、琼山、三亚的10个菌株为Mycosphaerella fijiensis,而白沙菌株既不是M.fijiensis,也不是M.musicola,其Mycosphaerella种的分类地位有待进一步研究。采用随机扩增多态DNA(RAPD)分析,将这些菌株区别为2类,支持PCR鉴定结果。本研究的鉴定结果对指导海南综合治理香蕉褐缘灰斑病的的实践有参考作用。

Identification of Nucleic Acid of Banana Sigatoka Leaf Spot Disease Pathogens in Hainan

Eleven isolates of banana Sigatoka leaf spot disease pathogens from Hainan were identified using the polymerase chain reaction (PCR) method and species-specific primers. The results showed that 10 of the 11 isolates from Danzhou, Ledong, Wenchang, Dongfan, Chenmai, Lingao, Qunhai, Changjiang, Qunsang and Sangya in Hainan were Mycosphaerellafijiensis, while the isolate from Baisha was neither M. fijiensis nor M. musicola. Further studies should be done to identify this isolate. Random amplified polymorphic DNA (RAPD) analysis showed that these isolates were in 2 groups, which supported the result of the PCR identification. The results of this study 'can be used as reference to guide the integrated management of banana Sigatoka leaf spot disease in Hainan.