同源多倍体萝卜过氧化物酶同功酶酶谱的研究

本文研究了染色体倍性与过氧化物酶同功酶的关系。为探讨染色体加倍的遗传效应提供有关生化方面的依据。试验用园盘式聚丙烯酰胺凝胶电泳法,对国光、旅大青萝卜的大,小花蕾和国光萝卜营养生长期叶片、叶脉中的过氧化物酶同功酶酶谱进行了分析。不同倍性的国光萝卜叶片、叶脉、大蕾、小蕾过氧化物酶同功酶酶谱均有相同的酶带、又有明显差异。以小蕾酶谱差异最显著。不同倍性的旅大青小蕾酶谱亦有明显差异。两个品种小蕾酶谱酶带数均以4 n 为最多,3 n 最少,2 n 居中。同一倍性的国光萝卜在不同发育阶段的不同器官中过氧化物酶同功酶酶谱具有明显差异。

STUDIES ON THE PEROXIDASE ISOZYME OF AUTOPOLYPLOID RADISH

Studies were made on the relationship between the ploidy levels of different varieties of radish(Raphanus sativus L.subsp hubernus alef.) and their respective peroxidase isozymes. Using the discpolyacrylamide gel electrophresis method,the peroxidase isozymes of flower buds,Ieaves,Ieaf veins of Guo-guang anp Luda-qing were analysed. It was observed that some of the peroxidase isozymes zymogram bands of the flowers,buds,leaves and leaf veins of Guo-guang and Luda- qing in different ploidy levels were similar....