| 玉米优良自交系转基因受体系统建立及转化后的筛选与再生 |
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| 引用本文： | 付凤玲,张莉萍,朱祯.玉米优良自交系转基因受体系统建立及转化后的筛选与再生[J].四川农业大学学报,2000,18(2):97-99. |
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| 作者姓名： | 付凤玲 张莉萍 朱祯 |
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| 作者单位： | 1. 四川农业大学,玉米研究中心,四川,雅安,625014
2. 中国科学院遗传研究所,北京,100101 |
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| 基金项目： | 四川省作物生物技术项目资助 |
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| 摘 要： | 通过对培养基和培养条件的筛选和改进 ,提高了诱导玉米优良自交系幼胚产生胚性愈伤组织 ,建立转基因受体系统的效率。通过比较试验 ,确定了潮霉素筛选的浓度和方法。PCR检测证明 ,目的基因已整合到玉米基因组中。
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| 关 键 词： | 玉米 转基因 受体 基因枪 |
The Establishment,Selection and Regeneration of Acceptor Systems with Excellent Inbreds in Maize |
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| FU Feng-ling,ZHANG Li-ping,ZHU Zhen.The Establishment,Selection and Regeneration of Acceptor Systems with Excellent Inbreds in Maize[J].Journal of Sichuan Agricultural University,2000,18(2):97-99. |
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| Authors: | FU Feng-ling ZHANG Li-ping ZHU Zhen |
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| Abstract: | The efficiency of inducing embryogenic calli from young embryos of excellent inbreds in maize and establishing transgenic acceptor systems is promoted through the selection and improvement of medium and culture conditions. The concentration and method of hygromycin selection are determined through contrast experiment. It is indicated by PCR assay that the aim gene have been integrated into maize genome. |
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| Keywords: | MAIZE TRANSGENIC ACCEPTOR GENE GUN |
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