马哈利樱桃砧木组培快繁技术研究

以马哈利樱桃砧木的嫩梢茎尖为外植体进行离体培养,筛选出的起始培养基为MS+6-BA 0．2- 0．5 mg／L;最佳增殖培养基为MS+6-BA 2．0 mg／L+IAA 0．5 mg／L+GA3 0．5 mg／L,此时增殖倍数达7．06,平均 苗高2．79 cm;最佳生根培养基为1／2 MS+IAA 2．0 mg／L或1／2 MS+IBA 0．6 mg／L,暗培养7 d后移至温室或 大棚,在自然光下生根炼苗,生根率达85％以上,单株平均根数3-6条,根系白嫩,组培苗生长整齐健壮;以营养土 为基质,用50 mg／L生根粉泥浆溶液处理生根组培苗的移栽成活率达87．0％以上。该技术体系适宜工厂化生产的 要求。

Study on rapid propagation technology of Prunus mahaleb

Vegetative axillary shoots were excised from field trees of Prunus mahaleb,then surfacesterilized and cultured on MS medium with 0. 2-0.5 mg/L 6-BA. The results showed that optimal medium for shoot proliferation were MS medium with 2.0 mg/L 6-BA and 0. 5 mg/L IAA and 0. 5 mg/L GA3. On this medium, the average propagation multiple was 7.06 and average height of plantlets was 2. 79 cm. Roots were induced on MS medium containing half-strength macroelements and 2. 0 mg/L IAA or 0. 6 mg/L IBA. After being cultured 7 days in the dark,axenic plantlets were transplanted to greenhouse for rooting and acclimation in the normal light ,more than 85 % plantlets were induced strong roots,the average number of roots each strain were 3-6. The plantlets dealt in slurry with 50 mg/L ABT1 were transplanted into nutrition soil and survival rate was more than 87.0 %. This propagation way can satisfy the need of factory production.