Characterization of a set of common wheat–Hordeum chilense chromosome 7Hch introgression lines and its potential use in research on grain quality traits

Chromosome 7H^ch from Hordeum chilense has potential for improving seed carotenoid content in wheat as it carries a Phytoene synthase 1 (Psy1) gene, which has a major role in this trait. Structural changes in chromosome 7H^ch were obtained in common wheat background by crossing the wheat disomic substitution line 7H^ch(7D) with a disomic addition line carrying chromosome 2C^c from Aegilops cylindrica in common wheat cv. ‘Chinese Spring’. Rearranged 7H^ch chromosomes were cytologically characterized by FISH. A set of 24 molecular markers and the Psy1 gene were used to identify the H. chilense chromosome segments involved in the introgressions. Six structural rearrangements of chromosome 7H^ch were identified. They included three homozygous wheat–H. chilense centromeric translocations, one involving the 7H^chS arm (T‐7H^chS·A/B) and two involving the 7H^chL arm (T1‐7H^chL·A/B and T2‐7H^chL·A/B). In addition, one 7H^chS arm deletion, one 7H^chL·7H^chL isochromosome and one 7H^chS telosome were obtained in hemizygous condition. These genetic stocks will be useful for studying the effect of chromosome 7H^ch on wheat flour colour.