近红外分析技术在玉米种质资源品质分析中应用

近红外分析技术具有快速、简便、准确、非破坏性的优点，为玉米品质育种提供了一个新的技术手段。利用FOSS公司1255型近红外透射光谱分析仪，对2000 年～2001年的686份玉米自交系和2001年～2002年的1438份杂交组合的籽粒品质成分进行测定。结果表明，玉米籽粒3种品质成分在不同材料间存在显著差异，粗脂肪和蛋白质变异幅度和变异系数都较大，粗淀粉含量变异幅度和变异系数都较小，年份之间有差异。自交系中粗脂肪平均含量分别为4.24%和4.27%，蛋白质平均含量分别为11.75%和12.17%，粗淀粉平均含量分别为71.04%和71.10%，玉米杂交组合粗脂肪平均含量分别为4.53%和4.86%，蛋白质平均含量分别为9.93%和10.05%，粗淀粉平均含量分别为72.56%和72.74%。

Study on application of the Near Infrared Spectroscopy(NIRS) in analyzing maize germplasm quality

NIRS was a new method for quality analyzer in crop breeding. The kernel quality of 686 maize inbred lines in 2000 year-2001year and 1438 cross combinations in 2001year-2002year were analyzed in this paper by using near infrared spectroscopy transmission (NIT) analyzer, which was made by FOSS Company. The heterosis of crude-fat, protein and crude starch in corn kernel and the relativity between hybrids and their parents was discussed also. It showed that the range of variation and the coefficient of variation were larger for crude-fat and protein content, the variance range and the coefficient of variation of crude-starch were lesser, the average content was 4.24%～4.27%,11.75%～12.17% and 71.04%～71.10% respectively for the crude-fat, protein and crude-starch in inbred lines, and was 4.53%～4.86%，9.93%～10.05% and 72.56%～72.74% respectively in cross combinations.