| 长期耕作对农田黑土几丁质降解菌群及酶活性的影响 |
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| 引用本文： | 隋鹏祥,罗洋,李瑞平,郑洪兵,袁野,郑金玉,王浩,刘武仁.长期耕作对农田黑土几丁质降解菌群及酶活性的影响[J].土壤学报,2023,60(6):1799-1809. |
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| 作者姓名： | 隋鹏祥 罗洋 李瑞平 郑洪兵 袁野 郑金玉 王浩 刘武仁 |
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| 作者单位： | 吉林省农业科学院农业资源与环境研究所/农业部东北作物生理生态与耕作重点实验室,吉林省农业科学院农业资源与环境研究所/农业部东北作物生理生态与耕作重点实验室,吉林省农业科学院农业资源与环境研究所/农业部东北作物生理生态与耕作重点实验室,吉林省农业科学院农业资源与环境研究所/农业部东北作物生理生态与耕作重点实验室,吉林省农业科学院农业资源与环境研究所/农业部东北作物生理生态与耕作重点实验室,吉林省农业科学院农业资源与环境研究所/农业部东北作物生理生态与耕作重点实验室,吉林省农业科学院农业资源与环境研究所/农业部东北作物生理生态与耕作重点实验室,吉林省农业科学院农业资源与环境研究所/农业部东北作物生理生态与耕作重点实验室 |
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| 基金项目： | 中国博士后科学基金资助项目（2021M693907）和吉林省农业科技创新工程项目（CXGC2020RCG010，CXGC2021RCB003） |
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| 摘 要： | 为揭示长期耕作对农田黑土几丁质降解菌群及酶活性的影响及其驱动因子，以黑土区耕法长期定位试验为平台，采用荧光定量和高通量测序技术研究不同耕作措施（灭茬起垄、免耕、间隔深松和翻耕）下0～40 cm土层chiA几丁质降解菌基因丰度、微生物群落结构和酶活性。结果表明：0～20 cm土层，免耕显著提高chiA基因丰度；20～40 cm土层，免耕降低chiA基因丰度、alpha多样性和变形菌门（Proteobacteria）相对丰度，但是增加放线菌门（Actinobacteria）相对丰度。土壤pH、平均重量直径和养分含量显著影响chiA基因丰度和群落结构。与灭茬起垄相比，免耕显著提高0～20cm土层几丁质酶活性，而间隔深松和深翻显著提高0～40 cm土层几丁质酶活性。结构方程模型模拟发现耕作方式、土壤深度、平均重量直径、土壤有机碳、全氮、全磷、chiA基因丰度和放线菌门相对丰度对几丁质酶活性具有显著直接效应。研究结果为明确黑土区不同耕作措施对土壤几丁质降解的影响提供理论基础。
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| 关 键 词： | 耕作措施 几丁质降解菌 chiA基因 几丁质酶活性 黑土 |
| 收稿时间： | 2022/3/18 0:00:00 |
| 修稿时间： | 2022/8/19 0:00:00 |
Effects of long-term different tillage practices on chitin degrading microbial communities and chitinase activity in farmland black soil |
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| Sui Pengxiang,Luo Yang,Li Ruiping,Zheng Hongbing,Yuan Ye,Zheng Jinyu,Wang Hao and Liu Wuren.Effects of long-term different tillage practices on chitin degrading microbial communities and chitinase activity in farmland black soil[J].Acta Pedologica Sinica,2023,60(6):1799-1809. |
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| Authors: | Sui Pengxiang Luo Yang Li Ruiping Zheng Hongbing Yuan Ye Zheng Jinyu Wang Hao and Liu Wuren |
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| Institution: | Institute of Agricultural Resource and Environment,Jilin Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Farming System in Northeast China,Ministry of Agriculture and Rural Affairs,Jilin,Institute of Agricultural Resource and Environment,Jilin Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Farming System in Northeast China,Ministry of Agriculture and Rural Affairs,Jilin,Institute of Agricultural Resource and Environment,Jilin Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Farming System in Northeast China,Ministry of Agriculture and Rural Affairs,Jilin,Institute of Agricultural Resource and Environment,Jilin Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Farming System in Northeast China,Ministry of Agriculture and Rural Affairs,Jilin,Institute of Agricultural Resource and Environment,Jilin Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Farming System in Northeast China,Ministry of Agriculture and Rural Affairs,Jilin,Institute of Agricultural Resource and Environment,Jilin Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Farming System in Northeast China,Ministry of Agriculture and Rural Affairs,Jilin,Institute of Agricultural Resource and Environment,Jilin Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Farming System in Northeast China,Ministry of Agriculture and Rural Affairs,Jilin,Institute of Agricultural Resource and Environment,Jilin Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Farming System in Northeast China,Ministry of Agriculture and Rural Affairs,Jilin |
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| Abstract: | |
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| Keywords: | Tillage practices Chitin degrading microorganism chiA gene Chitinase activity Black soil |
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