| 农杆菌介导几丁质酶基因转化南瓜的研究 |
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| 引用本文： | 王晶晶,屈淑平,崔崇士.农杆菌介导几丁质酶基因转化南瓜的研究[J].北方园艺,2007(10):184-185. |
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| 作者姓名： | 王晶晶 屈淑平 崔崇士 |
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| 作者单位： | 东北农业大学,园艺学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | 黑龙江省哈尔滨市科技攻关项目 |
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| 摘 要： | 选用"金辉一号"为试验材料,通过农杆菌介导成功地将菜豆几丁质酶基因导入到南瓜中,4株转化植株的PCR检测结果为阳性,扩增出900bp的目标带,初步证明几丁质酶基因整合到南瓜基因组中.
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| 关 键 词： | 南瓜 根癌农杆菌 几丁质酶基因 遗传转化 |
| 文章编号： | 1001-0009（2007）10-0184-02 |
| 收稿时间： | 2007-06-07 |
Introduction of Chitinase Gene Into Pumpkin By Agrobacterium-Mediated Transformation |
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| WANG Jing-jing,QU Shu-ping,CUI Chong-shi.Introduction of Chitinase Gene Into Pumpkin By Agrobacterium-Mediated Transformation[J].Northern Horticulture,2007(10):184-185. |
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| Authors: | WANG Jing-jing QU Shu-ping CUI Chong-shi |
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| Institution: | Horticultural College of Northeast Agricultural University, Harbin 150030,China |
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| Abstract: | The chitinase gene from kidney bean was introduced into Pumpkin Jinhui I by Agrobacterium mediated transformation,four transformed plants were positive and got 900 bp the target bands by PCR method and the gene has been introduced into the genome. |
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| Keywords: | Pumpkin Agrobacterium tumefaciens Chitinase gene Genetic-transformation |
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