| “青大一号”紫花苜蓿叶黄素提取研究 |
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| 引用本文： | 畅喜云,杨国柱. “青大一号”紫花苜蓿叶黄素提取研究[J]. 安徽农业科学, 2013, 41(11): 4855-4857 |
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| 作者姓名： | 畅喜云 杨国柱 |
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| 作者单位： | 青海畜牧兽医职业技术学院,青海湟源812100;甘肃农业大学草业学院草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070;青海大学,青海西宁,810000 |
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| 基金项目： | 青海省科技攻关项目,青海省自然科学基金 |
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| 摘 要： | [目的]从紫花苜蓿中提取叶黄素。[方法]以"青大一号"紫花苜蓿为材料,采取有机溶剂蒸馏萃取法,通过设置单因素提取剂(配比)、提取温度、料液比来考察对叶黄素提取工艺的影响。同时通过L9(34)正交试验来确定"青大一号"紫花苜蓿的最佳提取条件。[结果]提取叶黄素的最佳工艺条件为:选取乙醇和丙酮为混合提取剂,体积比2:1,料液比1.2∶50,提取温度55℃,此条件下提取叶黄素的含量为1.453 mg/L。[结论]从紫花苜蓿中提取叶黄素具有可行性。
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| 关 键 词： | 紫花苜蓿" 青大一号" 叶黄素 提取 |
Study on the Extraction of Lutein from Medicago sativa L.cv.Qingda 1 |
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| Affiliation: | CHANG Xi–yun et al(Qinghai Vocational and Technical College of Animal Husbandry and Veterinary Science,Huangyuan,Qinghai 812100) |
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| Abstract: | [Objective] The research aimed to extract lutein from alfalfa effectively,[Method] Using Medicago sativa L.cv.Qingda 1 as materials,single factor extraction solvent（ratio）,extraction temperature,material-liquid ratio were set up to study their effects on the extraction technology of lutein.The optimum extraction conditions of M.sativa L.cv.Qingda 1 were confirmed by using L9（34） orthogonal experiment.[Result] The optimum technology conditions for extracting lutein were as follows： selecting ethanol and acetone as mixed extraction solvent,volume ratio of 2∶1,material-liquid ratio of 1.2∶50,extraction temperature of 55 ℃.Under these conditions,lutein content was 1.453 mg/L.[Conclusion] It was feasible to extract lutein from alfalfa. |
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| Keywords: | Medicago sativa L.cv.Qingda No.1. Lutein Extraction |
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