Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time |
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| Authors: | Ben C Maddison John Spiropoulos Christopher M Vickery Richard Lockey Jonathan P Owen Keith Bishop Claire A Baker Kevin C Gough |
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| Affiliation: | .ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, UK ;.Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, UK ;.Current address: University of Southampton, Southampton, SO17 1BJ UK ;.School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, UK |
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| Abstract: | Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrPSc phenotypes were present: G338 or Apl338ii. Thirteen months later some divergent PrPSc phenotypes were seen: a mixture of Apl338ii with either G338 or P338, and a completely novel PrPSc deposition, designated Cag338. The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrPSc biological/biochemical properties. |
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