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Mode of Action of Diterpene and Characterization of Related Metabolites from the Soft Coral,Xenia elongata |
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| Authors: | Eric H. Andrianasolo Liti Haramaty Eileen White Richard Lutz Paul Falkowski |
| Affiliation: | 1.Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA; E-Mails: (E.H.A.); (L.H.); (R.L.);2.The Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, NJ 08903, USA; E-Mail: |
| Abstract: | Chemical and biological investigation of the cultured marine soft coral Xenia elongata led to the isolation of two new diterpenes (2, 3). Their structures were elucidated using a combination of NMR and mass spectrometry. Biological evaluations and assessments were determined using the specific apoptosis induction assay based on genetically engineered mammalian cell line D3 deficient in Bak and Bax and derived from a mouse epithelial cell. The diterpenes induce apoptosis in low micromolar concentrations. The results indicate that the previously isolated compound (1) affects cell in a manner similar to that of HSP90 and HDAC inhibitors and in a manner opposite of PI3 kinase/mTOR inhibitors. Compound (3) inhibits selectively HDAC6 in high micromolar concentrations. |
| Keywords: | structural and functional characterization of marine drug marine biotechnology bioactive compounds and bioproducts drug discovery and development |