| 辣椒RAPD反应体系建立及杂交种纯度鉴定研究 |
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| 引用本文： | 詹玉丝,陈晓,齐卫强,陈占宽,郅玉宝,杨春英,刘建民,郭善辉. 辣椒RAPD反应体系建立及杂交种纯度鉴定研究[J]. 辣椒杂志, 2004, 0(4): 32-35 |
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| 作者姓名： | 詹玉丝 陈晓 齐卫强 陈占宽 郅玉宝 杨春英 刘建民 郭善辉 |
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| 作者单位： | 河南省农业科学院;河南省农业科学院;河南省农业科学院 |
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| 基金项目： | 河南省科技攻关项目(1050120) |
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| 摘 要： | 以豫椒系列品种父母本和杂交种为材料，采用液氮-SDS法提取辣椒基因组DNA，使用Bio-RAD-Mycy-cler型PCR仪和Bio-RAD1000凝胶扫描仪，研究PCR反应的主要影响因子，建立适合辣椒RAPD分析的PCR反应体系，从40个10bp的随机引物中筛选出1个能鉴定豫椒968杂种纯度的引物S149。
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| 关 键 词： | 辣椒 DNA提取 杂种 纯度 RAPD分析 |
| 修稿时间： | 2004-08-16 |
Establishment of an Efficient RAPD System in Pepper and Its Application in Purity Testing of F1 Seeds |
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| Zhan Yusi Chen Xiao Qi Weiqiang Chen Zhankuan Zhi Yubao. Establishment of an Efficient RAPD System in Pepper and Its Application in Purity Testing of F1 Seeds[J]. Journal of China Capsicum, 2004, 0(4): 32-35 |
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| Authors: | Zhan Yusi Chen Xiao Qi Weiqiang Chen Zhankuan Zhi Yubao |
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| Affiliation: | Zhan Yusi1 Chen Xiao1 Qi Weiqiang1 Chen Zhankuan2 Zhi Yubao2 |
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| Abstract: | Hybrid chili pepper Yujiao series and their parents were adopted as the experimental materials in this experiment The genomic DNA was extracted with liquid N2- SDS method and PCR system that was suitable for RAPD analysis of these peppers was established.The results showed that primer S149 succeeded in testing the purity of hybrid pepper Yujiao 968 effectively. |
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| Keywords: | Capsicum(hot chili Peppers) hybrid purity DNA extraction RAPD analysis |
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