Increased apoptosis of peripheral blood mononuclear cells (PBMC) during general and epidural anaesthesia in dogs |
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Authors: | Galina P Simeonova Emil Slavov Roustislav Usunov Krasimira Halacheva Dinco N Dinev |
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Institution: | (1) Department of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria;(2) Department of Biology and Immunology, Medical Faculty, Trakia University, Stara Zagora, Bulgaria |
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Abstract: | The objective of the study was to investigate the hypothesis that perioperative lymphocytopenia was due to apoptosis of these
cells induced by either halothane or epidural anaesthesia in dogs. The relationship between apoptosis induction and plasma
concentrations of the stress hormone cortisol and the cytokines TNF-alpha and IL-10 was examined as well. The study was performed
on 22 healthy mongrel dogs, equal numbers from both genders, weighing 18.3 ± 2.9 kg, and aged between 3–5 years. Dogs were
divided in three groups. Eight of the animals were anaesthetized with halothane, another eight received epidural anaesthesia
using lidocaine, and six served as controls. Venous blood samples were obtained immediately before (0 minute) anaesthesia,
during deep anaesthesia (120 minute), and on the next day (24 hour) in order to determine the following parameters: the total
lymphocyte counts, the percentage of apoptotic peripheral blood mononuclear cells (PBMC) by flow cytometry, plasma concentrations
of the cytokines tumor necrosis factor – alpha (TNF-alpha) and interleukin – 10 (IL-10) by enzyme-linked immunosorbent assay
(ЕLISA), and plasma cortisol levels by radioimmune assay. Both halothane and epidural anaesthesia in dogs induces apoptosis
of PBMC with slight decrease in total lymphocyte counts. These immunomodulatory effects were transient and faded till the
24th hour. Concerning the mechanism of inducing lymphocyte apoptosis by general or epidural anaesthesia, it seemed that neither
cortisol, nor the tested cytokines TNF-alpha and IL-10 were implicated in this process. Further investigations are necessary
to confirm this assumption. |
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Keywords: | Apoptosis Dog Epidural anaesthesia Halothane PBMC |
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