A high-performance liquid chromatography method for determination of 4-aminopyridine in tissues and urine |
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Authors: | S W Casteel B R Thomas |
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Affiliation: | Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia 65205. |
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Abstract: | An analytical method was developed to measure 4-aminopyridine in tissues and urine to determine appropriate diagnostic samples in acute poisoning cases. Tissues from rats dosed with 4-aminopyridine were extracted with methylene chloride. Extracts were analyzed by high-performance liquid chromatography using an isocratic solvent system of acetonitrile and aqueous solution (15/85 v/v) consisting of 0.015 M sodium salt of l-heptane-sulfonic acid, 0.002 M tetramethylammonium bromide, and 0.01 M sodium dihydrogen phosphate adjusted to pH 3.0 with phosphoric acid. We concluded that suitable diagnostic samples for acute poisoning cases include stomach contents, kidney, liver, and urine. |
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