维生素B12、E和C在家畜精液保存中的应用

家畜精液保存时,向稀释液中添加一定量的维生素B12、E或C,在提高精液品质方面具有重要的作用。精液稀释液中添加维生素B12,具有维持精子顶体完整率,延长精子体外存活时间和提高解冻后的精子活力的作用。维生素E是重要的抗氧化剂,能通过抑制自由基的形成,阻止诱发的膜脂质发生过氧化,从而维持精子顶体膜的完整,提高精子顶体完整率和降低精子畸形率;维生素E还能防止精子发生冷休克,提高冻精解冻后的精子活力,从而提高受精率。维生素C作为抗氧化物质,能有效地防止冷冻精子磷脂发生过氧化,提高解冻后的精子活力,延长精子在体外存活的时间;维生素C的抗氧化作用还表现在能够保护精子细胞中的遗传基因DNA免受氧化破坏。

The Application of Vitamin B12, E and C in the Preservation of Livestock Semen

In the preservation of livestock semen,adding some vitamin B_(12),E or C in the diluted semen liquid has positive function to improve semen quality.Vitamin B_(12) can sustain sperm acrosome integrity,prolong its survival time in vitro,and increase sperm motility after thawing.Vitamin E,as an antioxidant,can prevent the superoxidate of the lipid membrane by inhibiting the formation of free radical,thereby to keep sperm acrosome integrity and lower the abnormal sperm rate;in addition,vitamin E also can prevent the cold shock of sperm,and increase sperm motility after thawing to improve sperm fertilization.As an antioxidant,vitamin C can prevent oxidation of sperm phosphorlipid effectively during the freezing period,improve the sperm motility after thawing and prolong the survival time in vitro.Vitamin C antioxidative effect shows in its ability to prevent DNA from destroying by oxidation.