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Metabolomic approach for age discrimination of Panax ginseng using UPLC-Q-Tof MS
Authors:Kim Nahyun  Kim Kemok  Choi Byeong Yeob  Lee Donghyuk  Shin Yoo-Soo  Bang Kyong-Hwan  Cha Seon-Woo  Lee Jae Won  Choi Hyung-Kyoon  Jang Dae Sik  Lee Dongho
Institution:School of Life Sciences and Biotechnology and ?Department of Statistics, Korea University , Seoul 136-713, Korea.
Abstract:An ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-Tof MS)-based metabolomic technique was applied for metabolite profiling of 60 Panax ginseng samples aged from 1 to 6 years. Multivariate statistical methods such as principal component analysis and hierarchical clustering analysis were used to compare the derived patterns among the samples. The data set was subsequently applied to various metabolite selection methods for sophisticated classification with the optimal number of metabolites. The results showed variations in accuracy among the classification methods for the samples of different ages, especially for those aged 4, 5, and 6 years. This proposed analytical method coupled with multivariate analysis is fast, accurate, and reliable for discriminating the cultivation ages of P. ginseng samples and is a potential tool to standardize quality control in the P. ginseng industry.
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