Liquid chromatographic determination of six sympathomimetic drugs in dosage forms

A simple and rapid stability-indicating liquid chromatographic method is described for quantitative determination of 6 sympathomimetic drugs in various liquid and solid formulations. Analyses were carried out on a C18 reverse phase column using 0.01M 1-octanesulfonic acid, sodium salt in 0.2% acetic acid-methanol (70 + 30) as the mobile phase with photometric detection at 220 nm. Coefficients of variation for 5 consecutive injections of a mixed standards solution ranged from 0.62% for metaraminol to 1.40% for epinephrine. Standard recoveries ranged from 98.8% for metaraminol to 100.8% for epinephrine. The method was linear between 0.2 and 10 micrograms of drug injected and was used successfully to analyze 17 commercial products in a variety of dosage forms.