| 红颜草莓叶片再生体系的建立 |
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| 引用本文： | 董莉,晁慧娟,董清华,金宝燕,高遐虹.红颜草莓叶片再生体系的建立[J].中国农学通报,2010,26(10):246-249. |
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| 作者姓名： | 董莉 晁慧娟 董清华 金宝燕 高遐虹 |
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| 作者单位： | 1. 北京农学院植物科学技术学院,北京,102206
2. 北京市海淀区西北旺镇农业综合服务中心,北京,100094 |
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| 基金项目： | 北京市教委科技发展计划 |
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| 摘 要： | 摘要：以红颜草莓 ( Fragaria ×ananassa Duch ‘Benihoppe’)组培苗为材料进行叶片离体再生研究，建立了一个高效的叶片再生体系。结果表明：暗培养10 d是叶片诱导愈伤组织最适合的暗培养时间。适宜的叶片诱导愈伤培养基为MS+TDZ 2.0mg/L +NAA 0.1mg/L，出愈率95.0%；适宜的愈伤诱导分化培养基为MS+TDZ 1.0mg/L +NAA 0.1mg/L，分化率90.0%；最适宜的生根培养基为1/2 MS，生根率为100%。
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| 关 键 词： | 高陡岩石边坡 高陡岩石边坡 人工客土基质 保水剂 土壤特性 |
| 收稿时间： | 2010-01-15 |
| 修稿时间： | 2010-03-15 |
Plant Regeneration System from Leaf Explants of Benihoppe Strawberry(Fragaria×ananassa Duch.) |
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| Dong Li,Chao Huijuan,Dong Qinghu,Jin Baoyan,Gao Xiahong.Plant Regeneration System from Leaf Explants of Benihoppe Strawberry(Fragaria×ananassa Duch.)[J].Chinese Agricultural Science Bulletin,2010,26(10):246-249. |
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| Authors: | Dong Li Chao Huijuan Dong Qinghu Jin Baoyan Gao Xiahong |
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| Institution: | 1Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206;
2Xi Bei Wang Agriculture Service Center, Beijing 100094 |
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| Abstract: | An efficient method for plant regeneration from leaf explant o‘f Benihoppe’strawberry （Fragaria × ananassa Duch） was established. The optimal callus inducing medium for Benihoppe’strawberry leaves was MS＋TDZ 2.0 mg/L ＋NAA 0.1 mg/L, and the callus induction rate was 95.0%.The optimal differentiation medium was MS＋TDZ1.0 mg/L＋NAA0.1 mg/L, and the differentiation rate was 90.0%. The optimal choice which could induce callus of the leaves was darkness treatment for 10 days. |
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| Keywords: | Benihoppe Strawberry tissue culture leaf regeneration dark treatment |
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