刺槐种子超低温保存研究

目的]为实现刺槐种质资源超低温保存提供理论和实践依据。方法]研究了刺槐种子的含水量、不同化冻方式及不同程度的机械撞击对超低温保存后刺槐种子的发芽率、电导率和脱氢酶活性的影响。结果]当刺槐种子的含水量从7.1%增加到9.1%,发芽率和TTCH含量明显降低,而电导率上升。这说明在保持种子活力的前提下,低含水量有利于刺槐种子的超低温保存。对于超低温保存后的刺槐种子,40℃温水化冻比慢速室温解冻更为适宜。机械撞击对处于超低温保存下的刺槐种子有一定的影响,故应尽量避免剧烈震动。结论]含水量对超低温保存后刺槐种子的发芽率影响显著。40℃温水化冻5min的化冻方式对超低温保存后刺槐种子的活力影响较小,种子发芽率较高。

Study on the Cryopreservation of Robinia pseudoacacia Seeds

Objective] The research aimed to provide theoretical and practical basis for realizing the cryopreservation of Robinia pseudoacacia germplasm resources. Method] The effects of water content in the seeds of R. pseudoacacia, different thawing ways and mechanical impact with different degree on the germination rate, electrical conductivity and dehydrogenase activity in the seeds of R. pseudoacacia after cryopreservation were studied. Result] When water content in the seeds of R. pseudoacaci was increased from 7.1 % to 9.1 %, the germination rate and TTCH content were obviously decreased, while the electrical conductivity was increased, which indicated that on the premise of keeping seed vigor,low water content was favorable for the cryopreservation of R. pseudoacacia seeds. For the seeds of R. pseudoacacia after cryopreservation,defrosting with 40 ℃ warm water was more favorable than slow thawing at room temperature. Mechanical impact had certain effects on R. pseudoacacia seeds under the conditions of cryopreservation, so strenuous vibration should be avoided if possible. Conclusion] Water content had significant effects on the germination rate of R. pseudoacacia seeds after cryopreservation. The thawing way of defrosting with 40 ℃ warm water for 5 min had little effect on the seed vigor of R. pseudoacacia seeds after cryopreservation and the germination rate of seeds was higher.