| 半番鸭Agouti基因的克隆与SSCP分析 |
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| 引用本文： | 缪中纬,朱志明,陈晖,郑嫩珠,李盛霖.半番鸭Agouti基因的克隆与SSCP分析[J].福建畜牧兽医,2011,33(1):6-8. |
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| 作者姓名： | 缪中纬 朱志明 陈晖 郑嫩珠 李盛霖 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所; |
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| 基金项目： | 福建省科技重大专项专题(2006NZ0003-3); 福建省农科院青年科技人才创新基金(2008QB-5)资助 |
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| 摘 要： | 以半番鸭血液基因组为模板进行PCR扩增,获得了半番鸭Agouti基因部分序列,该序列长为1178bp。序列分析表明该序列由部分第1外显子（92bp）、第1内含子（105bp）、完整的第2外显子（95bp）、完整的第2内含子（851bp）和部分第3外显子（35bp）组成;应用PCR-SSCP技术对扩增序列进一步研究发现位...
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| 关 键 词： | 半番鸭 Agouti基因 克隆 SSCP |
Cloning and SSCP analysis of mule duck Agouti gene |
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| Miao Zhongwei Zhu Zhiming Chen Hui Zheng Nenzhu Li Shenglin.Cloning and SSCP analysis of mule duck Agouti gene[J].Fujian Journal of Animal Husbandry and Veterinary,2011,33(1):6-8. |
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| Authors: | Miao Zhongwei Zhu Zhiming Chen Hui Zheng Nenzhu Li Shenglin |
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| Institution: | Miao Zhongwei Zhu Zhiming Chen Hui Zheng Nenzhu Li Shenglin (Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013) |
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| Abstract: | A pair of primer was designed to amplify the sequence of Agouti gene from mule duck genomic DNA.The fragment was cloned and sequenced which was 1 178 bp in length.The sequence analysis showed this fragment consisted of partial exon 1(92 bp),intron 1(105 bp),exon 2 (95 bp),intron 2 (851 bp)and partial exon 3(35 bp).Using PCR-SSCP for this amplified fragment,a C/T silent mutant was found at 80 bp in exon 2,which is useful for the further study on polymorphism and the correlation on duck feather color. |
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| Keywords: | mule duck AGOUTI gene clone SSCP |
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