|
|||||
|
|
| 内源酶辅助提取虾壳虾青素的研究 | |
| 引用本文： | 姜淼,杨贤庆,李来好,岑剑伟,郝淑贤,章超桦. 内源酶辅助提取虾壳虾青素的研究[J]. 南方水产, 2011, 7(2): 55-60. DOI: 10.3969/j.issn.2095-0780.2011.02.009 |
| 作者姓名： | 姜淼 杨贤庆 李来好 岑剑伟 郝淑贤 章超桦 |
| 作者单位： | 1. 广东海洋大学食品科技学院,广东,湛江,524088;中国水产科学研究院南海水产研究所,广东,广州,510300 2. 中国水产科学研究院南海水产研究所,广东,广州,510300 3. 广东海洋大学食品科技学院,广东,湛江,524088 |
| 基金项目： | 国家科技支撑计划项目(2008BAD94B02,2008BAD94B08); 广东省海洋渔业科技推广专项[A2009001-011(b),A200901B02,A200901I03]; 中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2010YD08,2010TS09) |
| 摘 要： | 文章探讨了内源酶对虾青素提取效果的影响,并以高效液相色谱法（HPLC）测定样品中虾青素的质量浓度,研究了pH、温度和酶解时间这3个因素对虾青素提取效果的影响,采用正交试验进行优化,以虾壳虾青素的萃取率作为评价提取效果的指标。结果表明,当酶解最佳工艺条件为pH 4.0,温度50℃,酶解时间1.5 h时,虾青素的萃取率最高,可达32.16μg.g-1湿虾壳,比直接超声提取提高28%。通过空白试验可知,外加脂肪酶对虾青素提取无促进作用,仅利用内源酶即可达到较好的提取效果,且经济节约。 |
| 关 键 词： | 虾壳 虾青素 内源酶 正交试验 |
Application of endogenous enzymes in astaxanthin extraction from shrimp shell |
|
| JIANG Miao,YANG Xianqing,LI Laihao,CEN Jianwei,HAO Shuxian,ZHANG Chaohua. Application of endogenous enzymes in astaxanthin extraction from shrimp shell[J]. South China Fisheries Science, 2011, 7(2): 55-60. DOI: 10.3969/j.issn.2095-0780.2011.02.009 | |
| Authors: | JIANG Miao YANG Xianqing LI Laihao CEN Jianwei HAO Shuxian ZHANG Chaohua |
| Affiliation: | JIANG Miao1,2,YANG Xianqing2,LI Laihao2,CEN Jianwei2,HAO Shuxian2,ZHANG Chaohua1(1.College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China,2.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China) |
| Abstract: | The extraction of astaxanthin from shrimp shell with endogenous enzyme is studied,and astaxanthin content in the extract is determined by high performance liquid chromatography（HPLC）.The pH,temperature and hydrolysis time are considered as the factors to study the extraction effect.Orthogonal test is adopted for optimization,taking the yield of astaxanthin from shrimp shell as the evaluation indicator.The results show that the optimum process conditions are 4.0 pH,50 ℃ and 1.5 h hydrolysis time,with the astaxanthin extraction from wet shrimp shell of 32.16 μg·g-1,which is 28% more than the extraction by direct ultrasonic treatment.No significant effect on the yield of astaxanthin is observed by adding lipase.Extracting astaxanthin by endogenous enzymes achieves better extraction effect and is economical. |
| Keywords: | shrimp shell astaxanthin endogenous enzyme orthogonal test |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |