|
|||||
|
|
| 转hpa1Xoo基因棉花活性氧产生及防卫相关基因的表达 | |
| 引用本文： | 缪卫国,宋从凤,乔子辰,王金生. 转hpa1Xoo基因棉花活性氧产生及防卫相关基因的表达[J]. 南京农业大学学报, 2011, 34(2) |
| 作者姓名： | 缪卫国 宋从凤 乔子辰 王金生 |
| 作者单位： | 1. 海南大学环境与植物保护学院,海南海口,570228 2. 南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室,江苏南京,210095 3. 新疆农业大学农学院,新疆乌鲁木齐,830052 |
| 基金项目： | 国家自然科学基金项目，国家973计划项目，国家科技支撑项目 |
| 摘 要： | 采用H2O2和酶活性检测、DAB染色、定量实时PCR等方法研究转hpalXoo基因棉花T-34叶组织中的活性氧.结果显示:无病原菌侵染时,T-34和出发品种Z35均未产生活性氧.在接种黄萎病菌分生孢子悬浮液后,与Z35相比,在T-34叶组织中接菌0-8 h H2O2含量变化呈现2个峰值;接菌8 h时PAL和POD酶活性显著增强;DAB染色显示褐红色斑产生;2个防卫基因ghAOX1和hsr203J超表达.这些结果表明,转hpalXoo基因棉花为某些防卫基因超水平表达提供了分子基础,使受到病原菌侵染时才诱导产生的高水平活性氧及相关基因表达,并且组成型表达的HarpinXoo赋予转基因棉花对黄萎病菌的抗性. |
| 关 键 词： | 转基因棉花 活性氧 防卫基冈 |
The generation of reactive oxygen intermediates and expression of defense genes in transgenic hpa1Xoo cotton |
|
| MIAO Wei-guo,SONG Cong-feng,QIAO Zi-chen,WANG Jin-sheng. The generation of reactive oxygen intermediates and expression of defense genes in transgenic hpa1Xoo cotton[J]. Journal of Nanjing Agricultural University, 2011, 34(2) | |
| Authors: | MIAO Wei-guo SONG Cong-feng QIAO Zi-chen WANG Jin-sheng |
| Affiliation: | MIAO Wei-guo1,SONG Cong-feng2,QIAO Zi-chen3,WANG Jin-sheng2(1.College of Environment and Plant Protection,Hainan University,Haikou 570228,China,2.College of Plant Protection/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,3.College of Agronomy Xinjiang Agricultural University,Urumqi 830052,China) |
| Abstract: | In this study,respective methods in detection of H2O2 and relative enzyme activity,DAB staining and quantitative real time PCR were used for confirming the reactive orygen intermediates(ROI)generation in T-34 compared with untransformed cotton Z35.The results showed that after T-34 leaves were inoculated by Verticillium dahliae conidia,compared with Z35 leaves,H2O2 content in T-34 leaf tissue exhibited two peak values during 0-8 h;PAL and POD activities were significantly enhanced,DAB staining revealed visu... |
| Keywords: | HarpinXoo PAL POD |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |