| 保加利亚精油玫瑰的离体起始培养与快速繁殖 |
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| 引用本文： | 康冰,荣生军,张金学,张广军. 保加利亚精油玫瑰的离体起始培养与快速繁殖[J]. 西北林学院学报, 2004, 19(1): 70-72 |
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| 作者姓名： | 康冰 荣生军 张金学 张广军 |
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| 作者单位： | 西北农林科技大学,生命科学学院,陕西杨陵,712100; 山东日照明珠园林工程公司,山东日照,276826; 甘肃省天祝县林业局,甘肃天祝,733200; 西北农林科技大学,资环学院,陕西杨陵,712100 |
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| 基金项目： | 国家林业局重点科学技术计划项目(97 4 09) |
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| 摘 要： | 以保加利亚精油玫瑰同一无性系相同部位茎段为试验材料,12～2月接种最好,芽生长迅速,污染率低,以6-BA,NAA,GA3及蔗糖为试验因子,每个因子5个含量水平,以不同培养基及浓度为基础,通过实验选出宜于保加利亚精油玫瑰组织培养的初代培养基,即MS+6-BA 2.0 mg/L+NAA0.15 mg/L+蔗糖40g/L+琼脂6g/L.
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| 关 键 词： | 保加利亚玫瑰 组织培养 增殖 适宜培养基 |
| 文章编号： | 1001-7461(2004)01-0070-03 |
| 修稿时间： | 2003-07-05 |
Tissue Culture and Rapid Reproduction of Rosa rugosa |
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| KANG Bing,RONG Sheng-jun,ZHANG Jin-xue,ZHANG Guang-Jun. Tissue Culture and Rapid Reproduction of Rosa rugosa[J]. Journal of Northwest Forestry University, 2004, 19(1): 70-72 |
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| Authors: | KANG Bing RONG Sheng-jun ZHANG Jin-xue ZHANG Guang-Jun |
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| Affiliation: | KANG Bing~1,RONG Sheng-jun~2,ZHANG Jin-xue~3,ZHANG Guang-Jun~4 |
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| Abstract: | A study on tissue culture and rapid reproduction of Rosa rugosa was carried out by using the stems with identical gene type and position. It has been found that the best inoculation period is from December to Febuary. New buds grew rapidly with the lowest rate of contamination. An orthogonal experiment was designed by using 6-BA, NAA, GA_3 and sucrose as factors with 5 concentration levels to select suitable preliminary medium: MS+6-BA 2.0 mg/L+NAA 0.15 mg/L+Sucrose 35 g/L+Agarophyte 6 g/L. |
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| Keywords: | Bulgarian Rosa rugosa culture multiply rapidly proper medium |
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