木耳菌糠对元蘑胞外酶活性的影响 |
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引用本文: | 邹莉,张国权,孙婷婷,王玥,杨苑艺.木耳菌糠对元蘑胞外酶活性的影响[J].安徽农业科学,2013(28):11269-11271,11324. |
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作者姓名: | 邹莉 张国权 孙婷婷 王玥 杨苑艺 |
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作者单位: | 东北林业大学,黑龙江哈尔滨,150040;东北林业大学,黑龙江哈尔滨,150040;东北林业大学,黑龙江哈尔滨,150040;东北林业大学,黑龙江哈尔滨,150040;东北林业大学,黑龙江哈尔滨,150040 |
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基金项目: | 黑龙江省博士后启动基金资助(LRB 1111762) |
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摘 要: | 目的]筛选利用木耳菌糠栽培元蘑的最佳配方。方法]设计4种配方,选取元蘑菌丝生长期、原基形成期和子实体发育期等阶段,测定其羧甲基纤维素酶(CMC)、半纤维素酶(HC)、淀粉酶(AMY)、漆酶(LAC)4种胞外酶的活性。结果]在不同配方中,元蘑菌丝体所分泌的CMC、HC、AMY和LAC酶活具有明显的差异,但同种酶的活性在不同配方中变化趋势基本一致;经过显著性分析,配方1、配方2和配方3胞外酶活性无显著差异,但配方4的酶活显著低于其他3种配考。结论]在胞外酶水平上,配方1、配方2和配方3用来栽培元蘑都是可行的。从经济成本考虑,配方3(茵糠含量为36%)为最佳配方。
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关 键 词: | 元蘑 木耳菌糠 胞外酶活性 |
Effect of Waste Material of Auricularla auricular on Hohenbuehelia serotina Extracellular Enzyme Activities |
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Institution: | ZOU Li et al (Northeast Forestry University, Harbin, Heilongjiang 150040) |
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Abstract: | Objective ] The research aimed to select the optimal formulation to cultivate Hohenbuehelia serotina with waste material of Auricu- laria auricular. Method ] 4 formulations were designed and mycetium growth phase, primordium formation phase and fruiting body growth phase were chosen, the activities of CMC, HC, AMY and LAC were determined. Result] In different formulations, CMC, HC, AMY and LAC that H. serotina mycelium secreted had significant differences, but the same enzyme activity had the consistent tendency in different for- mulations. After significance analysis, extracellular enzyme activities in formulation 1,2,3 had no significant differences, but they were signifi- cantly lower in formulation 4. Conclusion ] On the extracellular enzyme level, cultivating H. serotina with formulation 1,2,3 was feasible. Considering economic costs, formulation 3 (waste material of 36% ) was optimal. |
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Keywords: | Hohenbuehelia serotina Auricularia auricular Extracellular enzyme activities |
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