三聚氰胺在斑点叉尾鮰体内的残留及代谢规律研究

(21±1)℃水温条件下,研究了三聚氰胺在斑点叉尾鮰(Ictalurus punctatus)体内的残留消除规律。结果显示:血浆中药时数据符合有吸收一室开放模型,动力学方程为:C=3.952660(e-0.027279t-e-0.127279t),吸收半衰期(T1/2kα)为5.4469 h,消除半衰期(T1/2ke)为25.4093 h,达峰时间(Tp)为15.4045 h,达峰浓度(Cmax)为20.3985 mg/L,表观分布容积(Vd)为2.5763(mg/kg)/(mg/L)。肌肉、肝、肾中吸收半衰期(T1/2kα)分别为3.5582、4.1884、5.4397 h,消除半衰期(T1/2ke)为50.8081、23.3504、23.7242 h,达峰时间(Tp)为14.6766、12.6524、14.9967 h,达峰浓度(Cmax)为7.6449、22.9249、40.6047 mg/L,表观分布容积(Vd)为8.5657、2.3970、1.2712(mg/kg)/(mg/L)。结果表明:药物在体内吸收迅速,药物浓度较高,分布广泛,消除较为缓慢。以80 mg/kg剂量混饲口灌3 d后,各组织中三聚氰胺含量总体呈现肾脏>肝脏>肌肉。停止灌药后第5天肌肉中及第7天肝脏和肾脏组织中三聚氰胺含量低于我国(2008)卫生部公布的乳制品及含乳食品中三聚氰胺临时管理限量值和欧盟对中国进口产品设定了三聚氰胺的最大残留限2.5 mg/kg。

Metabolism and residues of melamine in the channel catfish(Ictalurus punctatus)

Metabolism and residues of melamine in Ictalurus punctatus were studied at water temperature of(21±1)℃.The results showed that pharmacokinetic data of I.punctatus in plasma confirmed to a one-compartment model with absorption in medicated feed administration.The pharmacokinetic in plasma equation was C=3.952660(e-0.027279t-e-0.127279t).The main pharmacokinetic parameters were absorption half-life(T1/2kα)=5.446949 h,elimination half-life(T1/2ke)=25.409327 h,Tp=15.404464 h,Cmax=20.398502 mg/L,apparent volume of distribution(Vd)=2.576271(mg/kg)/(mg/L).Elimination half-life in muscle,liver and kidney were 50.8081,23.3504,23.7242 h.The time to peak plasma concentration(Tp) were 14.6766,12.6524,14.9967 h.The peak concentration(Cmax) were 7.6449,22.9248,40.6047 mg/L.The apparent volume of distribution(Vd) were 8.5657,2.3970,1.2712(mg/kg)/(mg/L).The results showed:melamine were absorbed quickly in the fish,and it had high concentration,and distributed widely,while eliminated slowly.The results of residues showed that the content of melamine was kidney>liver>muscle.7 days after drug withdrawal,the melamine content in tissues was lower than the maximum residue limit of 2.5 mg/kg.