| 白芨繁殖技术研究 |
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| 引用本文： | 毛鑫,王锋,杨传桃,武芸.白芨繁殖技术研究[J].湖北农业科学,2017,56(20). |
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| 作者姓名： | 毛鑫 王锋 杨传桃 武芸 |
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| 作者单位： | 1. 湖北民族学院,生物科学与技术学院,湖北 恩施 445000;2. 湖北民族学院,生物资源保护与利用湖北省重点实验室,湖北 恩施 445000;3. 湖北民族学院,生物科学与技术学院,湖北 恩施 445000;湖北民族学院,生物资源保护与利用湖北省重点实验室,湖北 恩施 445000 |
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| 基金项目： | 湖北民族学院大学生创新训练计划项目，湖北省自然科学基金项目，湖北省教育厅科学技术研究项目 |
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| 摘 要： | 以名贵药材白芨Bletilla striata(Thunb.)Reichb.f.]种子萌发的无菌苗作为外植体进行组织培养,建立了白芨快速繁殖技术体系。结果表明,白芨种子萌发率最高的培养基为MS+1.6 mg/L 6-BA+0.3 mg/L NAA;诱导愈伤组织的最佳培养基为MS+1.0 mg/L 6-BA+1.5 mg/L 2,4-D;丛生芽增殖效果最明显的培养基为MS+1.0 mg/L 6-BA+0.15 mg/L NAA;0.5 mg/L GA3对白芨幼苗的生根作用最为明显,组培苗移栽以泥炭为基质时存活率和生长状况最佳。
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| 关 键 词： | 白芨[Bletilla striata(Thunb.)Reichb.f.] 种子 无菌苗 增殖 快速繁殖 |
Studies on the Rapid Propagation Technique of Bletilla striata |
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| MAO Xin,WANG Feng,YANG Chuan-tao,WU Yun.Studies on the Rapid Propagation Technique of Bletilla striata[J].Hubei Agricultural Sciences,2017,56(20). |
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| Authors: | MAO Xin WANG Feng YANG Chuan-tao WU Yun |
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| Abstract: | The sterile seedlings from seeds of Bletilla striata (Thunb.) Reichb. f. were used as explants for tissue culture and establish a rapid propagation technology system. As the study shows,the best culture medium for the sprouting of the B. stria-ta is as follows:MS+1.6 mg/L 6-BA+0.3 mg/L NAA;the best culture medium for the callus induction is as follows:MS+1.0 mg/L 6-BA+1.5 mg/L 2,4-D;the culture medium of the multiple shoots which has the most obvious propagation effect is as follows:MS+1.0 mg/L 6-BA+0.15 mg/L NAA;0.5 mg/L GA3 has the most distinct rooting effects on the B. striata seeding,the peat soil transplating substrate of blettila organizational seedings is the best. |
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| Keywords: | Bletilla striata (Thunb ) Reichb f seed sterile seedlings proliferation grapid production |
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