黄葵茎腐病病原菌的分离与鉴定

针对江苏宜兴黄葵主产区发生的黄葵茎腐病,通过分离黄葵植株不同部位(根、茎、叶)以及根际土壤、根围土壤的微生物,经形态学观察,结合分子进化分析构建了黄葵不同生态位的微生物物种系统发育树,对其种类和分布进行了分析。结果表明,细菌优势类群为芽胞杆菌,而真菌无明显优势类群。同时,根据柯赫氏法则测定了分离得到的微生物菌株的致病性,确定了菌株F28可引起黄葵茎腐病,经ITS分子进化分析及形态学观察,将其鉴定为变紫青霉Penicillium sanguineum。

Isolation and identification of the pathogen of Abelmoschus manihot stem rot

Root rot is an important disease of Abelmoschus manihot.By phylogenetic and morphological analysis of the microorganisms isolated from roots,stems,leaves and soil,we investigated the species and distribution of the microorganisms in different parts of A.manihot plant and rhizosphere soil from the main A.manihot-producing area in Yixing,Jiangsu Province.Meanwhile,we tested the pathogenicity of all the microorganisms based on Koch's postulates and determined that strain F28 could cause stem rot of A.manihot.The strain F28 was identified as Penicillium sanguineum according to its morphology and phylogenetic analysis of ITS.