Occurrence of soil insect pests: insight from classical identification supplemented with DNA barcoding |
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| Authors: | I Nyamwasa J Yin S Zhang J Kajuga A Hategekimana |
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| Institution: | 1. Agricultural Entomology, State Key Laboratory for Biology of Plant Disease and Insects Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China;2. Southern Agriculture Zone Divisions, Rwanda Agriculture Board, Kigali, Rwanda |
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| Abstract: | White grubs are key vegetables soil pests in Rwanda. However, aforementioned insect pests are unknown. We monitored adults with light traps and excavated larvae during cropping seasons of 2014 and 2015. Totally, 42 species were collected. The peak flights of key insect pests occurred from August to October and mid-March. The cytochrome C oxidase I (COI) gene was amplified with polymerase chain reaction (PCR) and used to compare larval DNA against DNA from adults by using MEGA 6, GMYC and ABGD software. The results obtained validate the use of COI gene. The identification results will help to predict critical time for management practices. |
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| Keywords: | Soil insect scarab beetles white grub cytochrome c oxidase I DNA barcoding |
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