Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate |
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| Authors: | Min-Kyoung Shin Myung Hwan Jung Won-Jung Lee Pil Son Choi Yong-Suk Jang Han Sang Yoo |
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| Affiliation: | 1Department of Infectious Disease, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.;2Department of Medicinal Plant Resources, Nambu University, Gwangju 506-824, Korea.;3Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Korea. |
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| Abstract: | Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection. |
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| Keywords: | Actinobacillus pleuropneumoniae ApxIIA cholera toxin B subunit protein transgenic plant |
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