| 高粱DNA导入水稻的初步研究 |
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| 引用本文： | 洪亚辉,任春梅,董延瑜,严钦泉,张学文.高粱DNA导入水稻的初步研究[J].湖南农业大学学报(自然科学版),1993(6). |
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| 作者姓名： | 洪亚辉 任春梅 董延瑜 严钦泉 张学文 |
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| 作者单位： | 湖南农学院生物技术系，湖南农学院生物技术系，湖南农学院生物技术系，湖南农学院生物技术系，湖南农学院生物技术系 长沙，410128，长沙，410128，长沙，410128，长沙，410128，长沙，410128 |
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| 摘 要： | 采用花粉管通道法,并配合仅剪1/3内颖及遮荫等措施,将高粱DNA导入水稻,获得以下结果:(1)共处理颖花367朵,收87粒种子,D_(?) 代结实率达23.7%;(2)D_1代出现变异,其中2株在穗型上明显倾向供体(高粱);(3)D_2代变异穗型出现分离,初步分析认为变异穗型为显性,受体类型为隐性,符合单基因分离的分离规律.
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| 关 键 词： | 水稻 高粱 外源 脱氧核糖核酸 基因转移/花粉管通道法 |
PRELIMINARY STUDIES ON THE INTRODUCTION OF SORGHUM DNA INTO RICE |
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| Hong yahui Ren Chunmei Dong yanyu Yan Qinquan Zhang Xuewen.PRELIMINARY STUDIES ON THE INTRODUCTION OF SORGHUM DNA INTO RICE[J].Journal of Hunan Agricultural University,1993(6). |
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| Authors: | Hong yahui Ren Chunmei Dong yanyu Yan Qinquan Zhang Xuewen |
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| Abstract: | Sorghum DNA was introduced into rice through rice pollen tube.Theexperimental results are as follows.(1)We have got 87 grains by treating 367 rice flowers.The setting percentage reached 23.7% on D_0 generation.(2)There are variant plants on D_1generation.Two variant plants among them resembled sorghum in appearance of apike.(3)The variant plant of spike appearance separates in AKD_2 generation.A Preliminarily analysissuggest that the separation of spike apperance accords with the seperation law of single geneand that the variety of spike apperance is dominant and the type of accepter was recessive. |
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| Keywords: | paddy sorghum exogenous dexyribonuleic acid transgenosis/method of pollen tabe passage |
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