鸭瘟病毒ZJ2016强毒株部分基因序列的测定与分析

2016年,浙江省一鸭场发生疑似鸭瘟病例,经鉴定,病鸭感染鸭瘟病毒,命名为ZJ2016病毒株。实验应用二代基因测序方法对该病毒核酸进行全基因测序,鉴定分析鸭瘟病毒ZJ2016株抗原与毒力相关基因序列特征。对测序病毒主要囊膜糖蛋白基因gB、gC、gG、gH、gI、gK、gL、gM、gN与致病力基因LORF11、UL2、UL12、UL41、UL47、US10进行核酸与氨基酸序列分析,并与已发表毒株序列及疫苗株(VAC株、clone-03株)序列比较。结果显示,与疫苗免疫保护抗原相关的ZJ2016株囊膜糖蛋白序列与疫苗毒株序列相比未发生明显的变异,而ZJ2016株的LORF11、UL2、UL12、UL41、UL47、US10基因结构及同源性与疫苗株氨基酸序列差异明显,且与中国其他分离强毒株LH2011株、CHv株、CV株同源性较高,表明该毒株具有强毒株的基因型特征。

Sequencing and analysis of several genes of a virulent duck enteritis virus strain ZJ2016

In 2016, a duck enteritis virus (DEV) strain ZJ2016 was identified in a duck farm infected with DEV in Zhejiang province. The antigen and virulence-related genes of ZJ2016 strain sequencing analysis was conducted using second-generation sequencer. The nucleic acid and amino acid sequences of the major envelope glycoprotein genes gB, gC, gG, gH, gI, gK, gL, gM, gN and pathogenicity genes LORF11, UL2, UL12, UL41, UL47, US10 were analyzed and compared with those of the published viruses and vaccine strains (VAC strain, clone-03 strain). The results showed that the sequence of ZJ2016 strain envelope glycoprotein associated with vaccine immune protection antigen did not change significantly compared with that of vaccine strain. However, the gene structure and homology of LORF11, UL2, UL12, UL41, UL47 and US10 of ZJ2016 strain were significantly different from the amino acid sequence of vaccine strain, and the homology of other virulent strains in China, such as LH2011, CHv and CV, were higher than that of vaccine strain. The results showed that ZJ2016 had the genotype characteristics of the virulent strain.