鸡源复合益生菌对青年白羽肉杂鸡免疫球蛋白和Toll样受体通路的影响

为研究鸡源复合益生菌对青年白羽肉杂鸡血清免疫球蛋白和肠道Toll样受体通路的影响,将180只健康的28日龄白羽肉杂鸡随机分为A(基础饲粮)、B(基础饲粮中添加10¹¹ CFU·kg^-1益生菌)、C(基础饲粮中添加2×10¹¹ CFU·kg^-1益生菌)3个处理,每个处理3个重复,每个重复20只鸡。试验期21 d。结果表明:与A组相比,B组和C组鸡血清中IgG、IgM水平显著(P<0.05)升高,B组和C组鸡肠道TLR2、TLR4、Myd88、TRAF-6、AP-1蛋白表达及mRNA相对表达量显著(P<0.05)升高。与B组相比,C组鸡血清IgG、IgM、IgA水平均无明显差异(P>0.05),鸡肠道TLR4、Myd88蛋白表达显著(P<0.05)升高,Myd88基因mRNA相对表达量显著(P<0.05)升高。综上,添加不同浓度的鸡源复合益生菌能够提高白羽肉杂鸡血清中IgG、IgM水平,通过Toll样受体通路增强肉鸡的免疫功能。

Effect of chicken-derived compound probiotics on immunoglobulin and toll-like receptor pathway of white feather broilers

To investigate the effects of probiotics on serum immunoglobulin and intestinal Toll-like receptor pathway in White feather broiler, one hundred and eighty 28-day-old white feather hybrid broilers were randomly divided into group I (basic diet), group II (basic diet adding 10¹¹ CFU·kg^-1 probiotics) and group III ((basic die adding 2×10¹¹ CFU·kg^-1 probiotics), with 3 repeats in each group and 20 broilers in each repeat. The experimental period was 21 d. The results showed that, compared with group A, the serum IgG and IgM levels in group B and group C increased significantly (P<0.05). The protein expression of TLR2, TLR4, Myd88, TRAF-6, AP-1 and their relative expression of mRNA in group B and group C were significantly (P<0.05) increased. Compared with group B, the serum IgG, IgM and IgA levels in group C were no significant difference (P>0.05). The protein expression of TLR4 and MyD88 in group C were significantly (P<0.05) increased, and the relative expression of MyD88 mRNA was significantly (P<0.05) increased. Feeding probiotics can improve the serum IgG and IgM levels of white feather broilers, and regulate the immune function of broiler intestine effectively through toll like receptor pathway.