太阳热处理对小白菜根肿病防治效果的研究

为探讨太阳热处理对小白菜根肿病的防治效果,以超级小黄白为供试材料,测定太阳热处理前后的土壤理化性质、根肿菌休眠孢子数量、土壤微生物多样性及发病率和病情指数,评价太阳热处理对小白菜根肿病的影响。研究表明:经太阳热处理后土壤0～10 cm处pH由4.96提高至5.60,根肿菌休眠孢子的浓度由1.09×10⁶ g^-1减少至5.74×10⁵ g^-1。变性梯度凝胶电泳(DGGE)结果证明,太阳热处理提高了土壤细菌微生物多样性,其中0～10 cm处和10～20 cm处土壤细菌微生物群落的香浓维纳指数分别由1.51、0.91提高到1.98、1.53,0～10 cm处根肿病的发病率由87.10%降低到49.12%,病情指数由45.24降低到16.96,防治效果明显。本研究为太阳热处理在实际农业生产中的应用及对根肿病的防治提供了理论基础。

Efficiency of soil solarization on control of Chinese cabbage clubroot

In order to investigate the effect of solar heat treatment on the Chinese cabbage clubroot disease, the variety of Chaojixiaohuangbai was used as the test material to determine the physical and chemical properties of the soil before and after solar heat treatment, the number of dormant spores, the microbial diversity and the incidence of disease and disease index of the roots. The results showed that the pH of the soil increased from 4.96 to 5.60 at 0-10 cm after heat treatment in the sun, and the concentration of dormant spores in the dry soil decreased from 1.09×10⁶ g^-1 to 5.74×10⁵ g^-1. The results of denaturing gradient gel electrophoresis (DGGE) showed that the solar heat treatment increased the bacterial microbial diversity of the soil. The Shannon-Wiener index of soil microbial community at 0-10cm and 10-20 cm increased from 1.51 and 0.91 to 1.98 and 1.53, respectively. The incidence of clubroot disease was reduced from 87.10% to 49.12%, and the disease index was reduced from 45.24 to 16.96. This study provided a theoretical basis for the application of solar heat treatment in actual agricultural production and the prevention of clubroot disease.