| 高产谷胱甘肽酵母菌株的筛选及其抗乙硫氨酸诱变研究(英文) |
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| 引用本文： | 冮洁,单立峰,吴耘红,张鹭. 高产谷胱甘肽酵母菌株的筛选及其抗乙硫氨酸诱变研究(英文)[J]. 农业科学与技术, 2008, 9(6): 35-38 |
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| 作者姓名： | 冮洁 单立峰 吴耘红 张鹭 |
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| 作者单位： | 冮洁,GANG Jie(大连民族学院生物技术与资源利用国家民委-教育部重点实验室,辽宁大连,116600);单立峰,吴耘红,张鹭,SHAN Li-feng,WU Yun-hong,ZHANG Lu(齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161001) |
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| 基金项目： | 辽宁省教育厅科研项目，大连民族学院博士启动基金 |
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| 摘 要： | [Objective] The aim of this study was to screen Saccharomyces for glutathione over-production. [Method] Ethionine-resistant mutants were obtained through UV mutagenesis and rational screening. [Result] A high GSH-producing strain HSJB1 was isolated from soil, and the biomass for this strain by flask shaking fermentation was 3.87 g/L while the GSH yield was 91.87 mg/L. According to the morphological, physiological and biochemical characteristics of cells, this strain was primarily identified as Saccharomyces cerevisiae. An ethionine-resistant mutant YBS77 was obtained through UV mutagenesis of the original strain HSJB1, and the biomass for this strain by flask shaking fermentation was 7.60 g dry cell weight/L while the GSH yield was 211.96 mg/L. [Conclusion] The biomass of the mutant obtained by breeding is increased by 96.38% than that of the original strain, and the GSH yield of the mutant obtained by breeding is increased by 130.72% than that from the original strain, which indicates that the breeding method is feasible.
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| 关 键 词： | Glutathione (GSH) Saccharomyces cerevisiae Strain breeding Mutant |
Screening of Saccharomyces Strains Highly Producing Glutathione and Breeding of Its Ethionine-resistant Mutants |
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| GANG Jie,SHAN Li-feng,WU Yun-hong,ZHANG Lu. Screening of Saccharomyces Strains Highly Producing Glutathione and Breeding of Its Ethionine-resistant Mutants[J]. Agricultural Science ＆ Technology, 2008, 9(6): 35-38 |
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| Authors: | GANG Jie SHAN Li-feng WU Yun-hong ZHANG Lu |
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| Affiliation: | 1. Key Laboratory of Biotechnology and Bioresources Utilization, the State Ethnic Affairs Commission, Ministry of Education, Dalian 116600;2. College of Life Science and Engineering, Qiqihar University, Qiqihar 161001 |
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| Abstract: | [Objective] The aim of this study was to screen Saccharomyces for glutathione over-production. [Method] Ethionine-resistant mutants were obtained through UV mutagenesis and rational screening. [Result] A high GSH-producing strain HSJB1 was isolated from soil, and the biomass for this strain by flask shaking fermentation was 3.87 g/L while the GSH yield was 91.87 mg/L. According to the morphological, physiological and biochemical characteristics of cells, this strain was primarily identified as Saccharomyces cerevisiae. An ethionine-resistant mutant YBS77 was obtained through UV mutagenesis of the original strain HSJB1, and the biomass for this strain by flask shaking fermentation was 7.60 g dry cell weight/L while the GSH yield was 211.96 mg/L. [Conclusion] The biomass of the mutant obtained by breeding is increased by 96.38% than that of the original strain, and the GSH yield of the mutant obtained by breeding is increased by 130.72% than that from the original strain, which indicates that the breeding method is feasible. |
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| Keywords: | Glutathione (GSH) Saccharomyces cerevisiae Strain breeding Mutant |
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