| 根癌农杆菌发酵生产辅酶Q10的培养条件优化 |
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| 引用本文： | 姜剑文,陈光,王立梅,朴金苗,齐斌.根癌农杆菌发酵生产辅酶Q10的培养条件优化[J].安徽农业科学,2010,38(12):6128-6130. |
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| 作者姓名： | 姜剑文 陈光 王立梅 朴金苗 齐斌 |
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| 作者单位： | 吉林农业大学生命科学学院,吉林长春,130118;常熟理工学院生物与食品工程学院,江苏常熟,215500 |
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| 摘 要： | 选用根癌农杆菌作为出发菌株,通过对其培养基优化来提高辅酶Q10产量。确定最佳培养基组成为：蔗糖35g/L,玉米浆50g/L,NaH2PO40.5%,Na2HPO40.5%,KNO30.5%。当发酵液初始pH值为7.5、接种量为12.5%,经32℃培养72h后辅酶Q10产量达到8.1mg/L,比最初条件增加了69.5%。
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| 关 键 词： | 根癌农杆菌 辅酶Q10 培养条件 |
Optimum Culture of Coenzyme Q10 Produced by Arobacterium tumefaciens during Fermentation Process |
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| Institution: | JIANG Jian-wen et al(College of Life Science,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | Arobacterium tumefaciens ATCC4452 was selected as the original strain.The production of Coenzyme Q10 was increased by optimizing the fermentation medium.The optimum fermentation medium contained sucrose 35 g/L,corn steep liquor 50 g/L,NaH2PO4 0.5%,Na2HPO4 0.5%,KNO3 0.5%.The optimum conditions for producing Coenzyme Q10 occured at initial pH 7.5,inoculum size 12.5% and 32 ℃.After 72 h fermentation,the production of Coenzyme Q10 was 8.1 mg/L,which increased 69.5% compare with the initial culture condition. |
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| Keywords: | Arobacterium tumefaciens Coenzyme Q10 Culture condition |
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