In vitro propagation of the African mahogany <Emphasis Type="Italic">Khaya senegalensis</Emphasis> |
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Authors: | Cao Dinh Hung Stephen J Trueman |
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Institution: | (1) School of Science and Education, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia;(2) Agri-Science Queensland, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia |
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Abstract: | A protocol was developed for shoot proliferation and plantlet formation of Khaya senegalensis, an important medicinal and timber plantation species introduced to Australia and southern Asia from western and central
Africa. We assessed effects of the plant growth regulators, benzyladenine, kinetin, naphthalene acetic acid and gibberellic
acid, on shoot proliferation and subsequent plantlet conversion. Shoot proliferation over four passages was higher in media
containing benzyladenine than in media containing other growth regulators, and optimal proliferation from seed of three different
sources was consistently obtained in medium containing 4.4 μM benzyladenine. Shoots from this medium were converted to plantlets
at high frequencies (76–90%) after treatment with 19.6 μM indole-3-butyric acid, and almost all plantlets were successfully
acclimatized to nursery conditions. These methods provide the means for establishing in vitro and ex vitro clone banks of
juvenile K. senegalensis trees for field selection of desired genotypes and tropical plantation establishment. |
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Keywords: | |
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