| 奶茶中三聚氰胺的HPLC测定方法 |
| |
| 引用本文： | 江和源,袁新跃,张建勇,崔宏春,刘晓辉,尧渝. 奶茶中三聚氰胺的HPLC测定方法[J]. 茶叶科学, 2009, 29(1) |
| |
| 作者姓名： | 江和源 袁新跃 张建勇 崔宏春 刘晓辉 尧渝 |
| |
| 作者单位： | 中国农业科学院茶叶研究所,农业部茶及饮料作物产品加工与质量控制重点实验室,浙江,杭州,310008;中国农业科学院茶叶研究所,农业部茶及饮料作物产品加工与质量控制重点实验室,浙江,杭州,310008;中国农业科学院研究生院,北京,100081 |
| |
| 基金项目： | 国家自然科学基金，浙江省钱江人才计划 |
| |
| 摘 要： | 建立了奶茶中三聚氰胺的HPLC测定方法。适用检测奶茶中三聚氰胺的HPLC条件为:色谱柱SunfireC18柱(250mm×4.6mmID:5μm),流动相由0.008mol/L辛烷磺酸钠和乙腈组成,柱温30℃,流速1.0ml/min,检测波长236nm,外标法定量。分析了市场上奶茶产品的三聚氰胺含量,发现少数奶茶产品中三聚氰胺的含量超过了国家暂行限量标准的2.5mg/kg。
|
| 关 键 词： | 高效液相色谱 奶茶 三聚氰胺 |
Determination of Melamine in Milk Tea By High Performance Liquid Chromatography |
| |
| JIANG He-yuan,YUAN Xin-yue,ZHANG Jian-yong,CUI Hong-chun,LIU Xiao-hui,YAO Yu. Determination of Melamine in Milk Tea By High Performance Liquid Chromatography[J]. Journal of Tea Science, 2009, 29(1) |
| |
| Authors: | JIANG He-yuan YUAN Xin-yue ZHANG Jian-yong CUI Hong-chun LIU Xiao-hui YAO Yu |
| |
| Affiliation: | 1. Key Laboratory of Tea and Beverage Crops Processing and Quality Control;Tea Research Institute;Chinese Academy of Agriculture Sciences;Hangzhou 310008;China;2. Graduate School;Beijing 100081;China |
| |
| Abstract: | The method to determinate melamine in milk tea by HPLC, was established. The samples were analyzed by a Sunfire C18 column (250 mm×4.6 mm ID; 5 μm). The mobile phase was composed of 0.008 mol/L sodium 1-Octanesulfonate (adjust to pH3.3 with acetic acid) and acetonitrile. The flow rate was 1.0 ml/min. Column temperature was 30℃. UV-detection was performed at 236 nm. The contents were quantified with exterior standard. Some milk tea samples from the supermarket were analyzed, and results showed that the content of melamine in some milk tea were higher than 2.5 mg/kg, the set limit in national standard. |
| |
| Keywords: | HPLC milk tea melamine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
